A selenoureido-iminoglycolipid transported by zeolitic-imidazolate framework nanoparticles: a novel antioxidant therapeutic approach.

A selenium-containing metal-organic framework with remarkable antioxidant capacity and ROS-scavenging activity was constructed by a controlled de novo encapsulation approach of a glycoconjugate mimetic, specifically a sp2-iminoglycolipid bearing a selenoureido fragment (DSeU), within a zeolitic-imidazolate framework exoskeleton. Biocompatible and homogeneous nanosized particles of ∼70 nm (DSeU@ZIF8) were obtained, which could be efficiently internalized in cells, overcoming the poor solubility in biological media and limited bioavailability of glycolipids. The ZIF-particle served as nanocarrier for the intracellular delivery of the selenocompound to cells, promoted by the acidic pH inside endosomes/lysosomes. As demonstrated by in vitro studies, the designed DSeU@ZIF8 nanoparticles displayed a high antioxidant activity at low doses; lower intracellular ROS levels were observed upon the uptake of DSeU@ZIF8 by human endothelial cells. Even more interesting was the finding that these DSeU@ZIF8 particles were able to reverse to a certain level the oxidative stress induced in cells by pre-treatment with an oxidizing agent. This possibility of modulating the oxidative stress in living cells may have important implications in the treatment of diverse pathological complications that are generally accompanied with elevated ROS levels.

Uremic Toxins Induce THP-1 Monocyte Endothelial Adhesion and Migration through Specific miRNA Expression.

Atherosclerosis is initiated by the activation of endothelial cells that allows monocyte adhesion and transmigration through the vascular wall. The accumulation of uremic toxins such as indoxyl sulphate (IS) and p-cresol (PC) has been associated with atherosclerosis. Currently, miRNAs play a crucial role in the regulation of monocyte activation, adhesion, and trans-endothelial migration. The aim of the present study is to evaluate the effect of IS and PC on monocyte adhesion and migration processes in monocytes co-cultured with endothelial cells as well as to determine the underlying mechanisms. The incubation of HUVECs and THP-1 cells with both IS and PC toxins resulted in an increased migratory capacity of THP-1 cells. Furthermore, the exposure of THP-1 cells to both uremic toxins resulted in the upregulation of BMP-2 and miRNAs-126-3p, -146b-5p, and -223-3p, as well as the activation of nuclear factor kappa B (NF-κB) and a decrease in its inhibitor IĸB. Uremic toxins, such as IS and PC, enhance the migratory and adhesion capacity of THP-1 cells to the vascular endothelium. These toxins, particularly PC, contribute significantly to uremia-associated vascular disease by increasing in THP-1 cells the expression of BMP-2, NF-κB, and key miRNAs associated with the development of atherosclerotic vascular diseases.

Vanadium oxide and a sharp onset of cold-trapping on a giant exoplanet.

The abundance of refractory elements in giant planets can provide key insights into their formation histories1. Owing to the low temperatures of the Solar System giants, refractory elements condense below the cloud deck, limiting sensing capabilities to only highly volatile elements2. Recently, ultra-hot giant exoplanets have allowed for some refractory elements to be measured, showing abundances broadly consistent with the solar nebula with titanium probably condensed out of the photosphere3,4. Here we report precise abundance constraints of 14 major refractory elements on the ultra-hot giant planet WASP-76b that show distinct deviations from proto-solar and a sharp onset in condensation temperature. In particular, we find nickel to be enriched, a possible sign of the accretion of the core of a differentiated object during the evolution of the planet. Elements with condensation temperatures below 1,550 K otherwise closely match those of the Sun5 before sharply transitioning to being strongly depleted above 1,550 K, which is well explained by nightside cold-trapping. We further unambiguously detect vanadium oxide on WASP-76b, a molecule long suggested to drive atmospheric thermal inversions6, and also observe a global east-west asymmetry7 in its absorption signals. Overall, our findings indicate that giant planets have a mostly stellar-like refractory elemental content and suggest that temperature sequences of hot Jupiter spectra can show abrupt transitions wherein a mineral species is either present or completely absent if a cold trap exists below its condensation temperature8.

La evaluación del involucramiento cultural en personas indígenas: escala para un pueblo andino / The evaluation of cultural involvement in indigenous people: scale for an Andean people

Objetivo: diseñar y evaluar las propiedades psicométricas de una escala para medir el involucramiento cultural en un pueblo andino. Metodología: investigación psicométrica. Fueron desarrolladas las fases de: a) revisión teórica; b) abordaje cualitativo, entrevistas semiestructuradas a 16 Lickanantay; c) diseño de la escala preliminar; d) evaluación cognitiva (5 Lickanantay); e) evaluación psicométrica con 200 Lickanantay, siendo 124 mujeres (62%) y 76 hombres (38%). Resultados principales: la escala quedó constituida por 10 ítems que identifican el involucramiento cultural a partir de la participación en actividades tradicionales Lickanantay como festividades y ceremonias. El análisis factorial entregó una solución unifactorial con un α de Cronbach de 0.924 y los índices de bondad de ajuste dan cuenta de un ajuste excelente del modelo. Conclusión principal: La escala permitirá evaluar el efecto y relación del involucramiento cultural en la salud y el bienestar de personas y comunidades del pueblo andino Lickanantay.(AU)

Objective: to design and evaluate the psychometric properties of a scale to measure cultural involvement in an Andean town. Methodology: psychometric research. The following phases were developed: a) theoretical review; b) qualitative approach, semi-structured interviews with 16 Lickanantay; c) preliminary scale design; d) cognitive evaluation (5 Lickanantay); e) psychometric evaluation with 200 Lickanantay, being 124 women (62%) and 76 men (38%). Main results: the scale was made up of 10 items that identify cultural involvement from participation in traditional Lickanantay activities such as festivities and ceremonies. The factorial analysis provided a unifactorial solution with a Cronbach's α of 0.924 and the goodness of fit indices show an excellent fit of the model. Main conclusion: The scale will allow evaluating the effect and/or relationship of cultural involvement in the health and/or well-being of people and/or communities of the Lickanantay Andean people.(AU)

Validación de la versión en español de la escala perspectiva espiritual de P. Reed en personas mayores, Chile / Validation of the spanish version of P. Reed's spiritual perspective scale in older people in Chile

El envejecimiento global es uno de los grandes desafíos sanitarios del siglo XXI, el 2030 una de cada seis personas tendrá ≥ 60 años. En Chile se proyecta un 32,1% para el 2050. Esta realidad demográfica desafía a la enfermería en el abordaje multidimensional del envejecimiento. La espiritualidad como atributo de la autotrascendencia es un factor condicionante de acciones promotoras de salud en las personas mayores. Desde la perspectiva de la Dra. Reed, se presentan los resultados de validación de su Escala de Perspectiva Espiritual. La población fue de 202 personas mayores en promedio de 71 años (D.E.= 8,2338). El método utilizado fue diseño psicométrico y corte transversal. La escala general presentó; α de Cronbach=0,92; dimensiones prácticas espirituales α de Cronbach= 0,822; dimensión creencias espirituales α de Cronbach=0,914. El análisis de modelo de un factor (CFI=0,956, RMSEA=0,167 IC95% [0,147 - 0,188] y un c2=232,762; p=0,000), como de dos factores coherentes con la estructura teórica propuesta por Reed (CFI=0,984; RMSEA=0,102 IC95% [0,081 ­ 0,125] y c2=105,890; p=0,000), presentaron cargas factoriales altas y relativamente homogéneas. La escala general y las subescalas de creencias y prácticas espirituales mostraron adecuada confiabilidad. El análisis de modelos anidados mostró un mejor ajuste del modelo de dos factores. Los resultados suman para afirmar que la Escala de Perspectiva Espiritual, contribuye a la precisión empírica, potencial heurístico y verificabilidad de la Teoría de Transcendencia de Reed. Se vislumbra que la espiritualidad es un factor mediador en la relación entre la vulnerabilidad, autotrascendencia y bienestar de la persona mayor.

Global aging is one of the great health challenges of the 21st century. By 2030, one in six people will be ≥60 years of age, with 32.1% projected for Chile by 2050. This demographic reality challenges nursing in the multidimensional approach to aging. The dimension of spirituality as an attribute of self-transcendence is a conditioning factor of health promoting actions in the elderly. The validation results of Dr. Reed's Spiritual Perspective Scale are presented. The population included 202 people older than 71 years on average (SD= 8.2338). The method used was a psychometric and cross-sectional design. The general scale presented Cronbach's α=0.92; spiritual practical dimension, Cronbach's α=0.822; spiritual beliefs dimension, Cronbach's α=0.914. The analysis of a one-factor model (CFI=.956, RMSEA=0.167 IC95% [0.147-0.188] and c2=232.762; p=0.000), as two factors consistent with the theoretical structure proposed by Reed (CFI=0.984; RMSEA=0.102 IC95% [0.081-0.125] and c2=105.890; p=0.000), presented high and relatively homogeneous factorial loads. The general scale and the subscales of spiritual beliefs and practices showed adequate reliability. The analysis of nested models showed a better adjustment of the two-factor model. The results collectively affirm that the Spiritual Perspective Scale contributes to the empirical precision, heuristic potential, and verifiability of Reed's Theory of Transcendence. It is observed that spirituality is a mediating factor in the relationship between vulnerability, self-transcendence, and well-being of humans.

Efecto mediador de la motivación a buscar venganza en la relación de la ansiedad y la evitación en el apego con la calidad de vida / Mediating effect of revenge-seeking motivation on the relationship of attachment anxiety and avoidance to quality of life

OBJETIVO: Determinar el efecto mediador de la motivación a buscar venganza en la relación de la ansiedad y la evitación en el apego con la calidad de vida. METODOLOGÍA: estudio transversal correlacional. La muestra estuvo constituida por 558 personas de 18 a 65 años de la ciudad de Antofagasta, Chile. Fueron utilizados los instrumentos: Cuestionario WHOQoL-Bref; Experiencia en Relaciones Cercanas; y Motivación a buscar venganza. Se realizó un modelo de ecuaciones estructurales que consideró efectos directos, indirectos y totales. RESULTADOS: Del total de participantes, 289 (51,8%) fueron hombres. Las edades fluctuaron entre 18 y 65 años (M=39,7; ±13,42). Se encontraron efectos significativos en la relación entre la ansiedad y evitación en el apego en los dominios físico, psicológico y social-relacional de la calidad de vida. La motivación a buscar venganza tiene efecto de mediación total en la relación entre evitación en el apego y el dominio físico, y efecto de mediación parcial de la evitación en el apego sobre el dominio psicológico de la calidad de vida. CONCLUSIÓN: ansiedad y evitación en el apego disminuyen la calidad de vida y la motivación a buscar venganza media parcial o totalmente la relación entre la evitación en el apego sobre los dominios físico y psicológico de la calidad de vida.

OBJECTIVE: To determine the mediating effect of revenge-seeking motivation on the relationship between anxiety and avoidance in attachment and quality of life. METHODOLOGY: cross-sectional correlational study. The sample consisted of 558 people aged 18 to 65 years from the city of Antofagasta, Chile. The following instruments were used: WHOQoL-Bref Questionnaire; Experience in Close Relationships; and Motivation to seek revenge. Structural equation modeling was performed considering direct, indirect and total effects. RESULTS: Of the total number of participants, 289 (51.8%) were male. Ages ranged from 18 to 65 years (M=39.7; ±13.42). Significant effects were found in the relationship between anxiety and avoidance on attachment in the physical, psychological, and social-relational domains of quality of life. Revenge-seeking motivation has full mediation effect on the relationship between attachment avoidance and the physical domain, and partial mediation effect of attachment avoidance on the psychological domain of quality of life. CONCLUSION: Anxiety and attachment avoidance decrease quality of life, and revenge-seeking motivation partially or fully mediates the relationship between attachment avoidance and the physical and psychological domains of quality of life.

Predictores demográficos y psicosociales de salud fisica percibida por migrantes colombianos y peruanos en chile

Objetivo: Identificar la capacidad predictiva de variables demográficas y psicosociales en el autorreporte de la salud física de migrantes colombianos y peruanos en Chile. Material y Método: Diseño no experimental de tipo transversal, correlacional, con muestreo intencional. Participaron 853 inmigrantes (51,7% colombianos y 48,3% peruanos; 51% mujeres) entre 18 a 71 años. Se evaluó el autorreporte del estado de salud física, variables sociodemográficas, síntomas ansiosos y depresivos, estrés por aculturación, apoyo social percibido, autoestima individual, entre otros, los que fueron analizados mediante regresión múltiple. Resultados: La población colombiana reportó un mejor estado de salud física. En ambos grupos, los síntomas ansiosos y depresivos fueron importantes predictores del estado de salud física. Otras variables predictivas fueron en peruanos: ingresos económicos, distancia con el país origen y años de permanencia en Chile, en tanto en colombianos: autoestima individual, discriminación y rechazo, y estado de salud actual en comparación con el que tenían en su país. En colombianos, el modelo explica el 14,7% de la varianza, en peruanos un 28,9%. Conclusiones: En ambas poblaciones los síntomas ansiosos y depresivos, y el estrés por aculturación resultaron ser importantes predictores del autorreporte de la salud física, aportando evidencia a que el proceso migratorio afecta el estado de salud mental del migrante y este, a su vez, puede afectar su estado de salud física.

Objective: To identify the predictive capacity of demographic and psychosocial variables in the self-reported physical health of Colombian and Peruvian migrants in Chile. Materials and Methods: Non-experimental, cross-sectional, correlational design using intentional sampling. A total of 853 immigrants (51.7% Colombians and 48.3% Peruvians; 51% women) between 18 and 71 years of age participated. Self-reported physical health status, sociodemographic variables, anxious and depressive symptoms, acculturation stress, perceived social support, individual self-esteem, among others, were evaluated and analyzed using multiple regression. Results: The Colombian population reported a better state of physical health. In both groups, anxious and depressive symptoms were important predictors of the physical health status. Other predictive variables were among Peruvians: income, distance from country of origin and years of permanence in Chile, while among Colombians: individual self-esteem, discrimination and rejection, and current health status compared to the one they had in their country. Among Colombians, the model explains 14.7% of the variance, whereas among Peruvians it accounts for 28.9%. Conclusions: In both populations, anxious and depressive symptoms, as well as acculturation stress turned out to be important predictors of self-reported physical health, providing evidence that the migratory process affects the migrant's mental health status, and this in turn can affect their physical health status.

Objetivo: Identificar a capacidade preditiva das variáveis demográficas e psicossociais na saúde física autorreferida de migrantes colombianos e peruanos no Chile. Material e Método: Desenho não experimental, transversal e correlacional com amostragem intencional. Participaram 853 imigrantes (51,7% colombianos e 48,3% peruanos; 51% mulheres) entre 18 e 71 anos. O estado de saúde física autorreferido, variáveis sociodemográficas, sintomas ansiosos e depressivos, estresse de aculturação, apoio social percebido, auto-estima individual, entre outros, foram avaliados e analisados por meio de regressão múltipla. Resultados: A população colombiana relatou um melhor estado de saúde física. Em ambos os grupos, sintomas ansiosos e depressivos foram importantes preditores do estado de saúde física. Outras variáveis preditoras foram entre os peruanos: renda económica, distância do país de origem e anos de permanência no Chile, enquanto nos colombianos: auto-estima individual, discriminação e rejeição, e estado de saúde atual em comparação ao que tinham em seu país. Entre os colombianos, o modelo explica 14,7% da variância, entre os peruanos ele representa 28,9%. Conclusões: Em ambas as populações, os sintomas ansiosos e depressivos e o estresse de aculturação revelaram-se importantes preditores da saúde física autorreferida, evidenciando que o processo migratório afeta o estado de saúde mental do migrante, e este, por sua vez, pode afetar seu estado de saúde física.

Passage Number-Induced Replicative Senescence Modulates the Endothelial Cell Response to Protein-Bound Uremic Toxins.

Endothelial aging may be induced early in pathological situations. The uremic toxins indoxyl sulfate (IS) and p-cresol (PC) accumulate in the plasma of chronic kidney disease (CKD) patients, causing accelerated endothelial aging, increased cardiovascular events and mortality. However, the mechanisms by which uremic toxins exert their deleterious effects on endothelial aging are not yet fully known. Thus, the aim of the present study is to determine the effects of IS and PC on endothelial damage and early senescence in cultured human umbilical vein endothelial cells (HUVECs). Hence, we establish an in vitro model of endothelial damage mediated by different passages of HUVECs and stimulated with different concentrations of IS and PC to evaluate functional effects on the vascular endothelium. We observe that cell passage-induced senescence is associated with apoptosis, ROS production and decreased endothelial proliferative capacity. Similarly, we observe that IS and PC cause premature aging in a dose-dependent manner, altering HUVECs' regenerative capacity, and decreasing their cell migration and potential to form vascular structures in vitro. In conclusion, IS and PC cause accelerated aging in HUVECs, thus contributing to endothelial dysfunction associated with CKD progression.

Ethnic Identity and Well-Being of Andean Indigenous People: The Effect of Individualistic and Collectivist Value Orientations.

The aim of this research was to evaluate the mediating effect of the value orientations of collectivism and individualism on the relationship between ethnic identity and well-being, the latter conceived from the worldview of Andean natives. For this purpose, under an observational and cross-sectional design, 395 Lickan-Antay adults (57% women) living in areas of indigenous development and in two cities in northern Chile were surveyed. We used the Lickan-Antay BLA32 well-being scale, a short version of the Portrait 21 Values Questionnaire to measure individualistic and collectivistic values, and an adapted version of the Ethnic Identity Scale. The results show that ethnic identity had a direct positive effect on all three dimensions of well-being (harmony with community life, ethnic harmony and harmony with nature), and total indirect effects on all five dimensions of well-being, one of them originating mainly from collectivist orientations. Individualistic orientations also showed a positive, though less intense, mediating effect on well-being. We conclude that collectivist and individualistic motivational patterns coexist in Lickan-Antay natives and explain an important part of the relationship between ethnic identity and well-being. Finally, we discuss our results and suggest replication of this study in other ethnic contexts to assess the generalizability of these findings to other native peoples of the Andean region of South America.

Compasión, celos y envidia: emociones sociales y cyberbullying entre adolescentes / Compassion, jealousy and envy: social emotions and cyberbullying among adolescents

Las emociones cumplen un papel fundamental en la capacidad de acción de los seres humanos, razón por la cual es indispensable estudiarlas para entender los fenómenos sociales contemporáneos en los que participan los adolescentes. Esta investigación tiene como objetivo comprender las experiencias de la compasión, los celos y la envidia de los adolescentes implicados en situaciones de cyberbullying en los roles de espectadores y agresores. Se trata de una investigación con diseño cualitativo en la que se entrevistó a 31 adolescentes entre los 12 y 17 años (M=15,23, DS=1,6) que participaron de situaciones de cyberbullying como espectadores y/o agresores. Los resultados indican que la compasión adquiere la forma de tristeza y rabia, especialmente entre los espectadores de cyberbullying. Por otra parte, los celos son un motivo que justifica la agresión a otros a través de Internet, y una forma de gestionar las tensiones en los vínculos sexo-afectivos. El uso de redes sociales digitales y la participación en escenarios presenciales en los que se disputan posiciones a nivel académico favorece el proceso de comparación social vinculado a la envidia que está en la base del cyberbullying. Finalmente, este estudio es una invitación para padres, docentes e institucionales estatales a que acompañen a los adolescentes a gestionar sus conflictos sin que las agresiones presenciales y online medien en sus interacciones

Emotions play a fundamental role in the capacity for action of human beings, which is why it is essential to study them in order to understand contemporary social phenomena in which adolescents participate. This research aims to understand the experiences of compassion, jealousy and envy of adolescents involved in cyberbullying situations in the roles of bystanders and aggressors. This is a qualitative design research in which 31 adolescents between the ages of 12 and 17 (M=15.23, SD=1.6) who participated in cyberbullying situations as bystanders and aggressors were interviewed. The results indicate that compassion takes the form of sadness and anger, especially among cyberbullying bystanders. On the other hand, jealousy is a motive that justifies aggression to others through the Internet, and a way of managing tensions in sex-affective bonds. The use of digital social networks and participation in face-to-face scenarios in which positions are disputed at the academic level, favours the process of social comparison linked to envy that is at the basis of cyberbullying. Finally, this study is an invitation for parents, teachers and state institutions to accompany adolescents in managing their conflicts without face-to-face and online aggressions mediating their interactions.

El significado del bienestar desde la perspectiva de un pueblo originario andino: lickan-antay / Meaning of well-being from the lickan-antay perspective: a qualitative and decolonialist approach / O significado do bem-estar a partir da perspectiva de um povo nativo andino: lickan-antay

OBJETIVO: Analizar el significado del bienestar desde la perspectiva de un pueblo indígena andino: lickan-antay. MÉTODO: Estudio cualitativo, diseño descriptivo con fines comprensivos, con muestreo intencionado por bola de nieve. Se aplicaron entrevistas semiestructuradas, analizando los discursos de 16 lickan-antay de diferentes comunidades del norte de Chile, utilizando para esto, herramientas de la teoría fundamentada. RESULTADOS: El significado del bienestar para los/las lickan-antay, se relaciona con la integración de diferentes armonías: armonía interna, armonía comunitaria, armonía con la naturaleza y la armonía en el desarrollo étnico-cultural lickan-antay. CONCLUSIONES: El significado del bienestar para los/las lickan-antay está orientado por una concepción del mundo eco-cosmocéntrica, compartida con otros pueblos indígenas andinos, que difiere de los enfoques occidentales del bienestar. Se hace necesario desarrollar una línea de investigación local, que permita establecer un cuerpo de conocimientos transculturales, en torno cuidado y la atención de las/los lickan-antay

OBJECTIVE: To analyze the meaning of well-being from the perspective of an Andean indigenous people: lickan-antay. Method. Qualitative study, descriptive design for comprehensive purposes, with intentional snowball sampling. Semi-structured interviews were applied, analyzing the speeches of 16 lickan-antay from different communities in northern Chile, using tools of grounded theory. RESULTS: The meaning of well-being for the lickan-antay is related to the integration of different harmonies: internal harmony, community harmony, harmony with nature and harmony in the ethnic-cultural development of lickan-antay. CONCLUSIONS: The meaning of well-being for the Lickan-antay is guided by a conception of the eco-cosmocentric world, shared with other indigenous Andean peoples, which differs from Western approaches to well-being. It is necessary to develop a line of local research, which allows establishing a body of cross-cultural knowledge, around the care and attention of the Lickan-Antay

OBJETIVO: Analisar o significado do bem-estar na perspectiva de um povo indígena andino: lickan-antay. MÉTODO: Estudo qualitativo, descritivo, para fins abrangentes, com amostragem intencional de bolas de neve. Entrevistas semi-estruturadas foram aplicadas, analisando os discursos de 16 lickan-antay de diferentes comunidades do norte do Chile, utilizando ferramentas da teoria fundamentada. RESULTADOS: O significado do bem-estar para o lickan-antay está relacionado à integração de diferentes harmonias: harmonia interna, harmonia comunitária, harmonia com a natureza e harmonia no desenvolvimento étnico-cultural de lickan-antay. CONCLUSÕES: O significado de bem-estar para o Lickan-antay é guiado por uma concepção do mundo eco-cosmocêntrico, compartilhado com outros povos indígenas andinos, que difere das abordagens ocidentais ao bem-estar. É necessário desenvolver uma linha de pesquisa local, que permita estabelecer um corpo de conhecimento intercultural, em torno do cuidado e atenção do Lickan-Antay

Inflammation, Senescence and MicroRNAs in Chronic Kidney Disease.

BACKGROUND: Patients with chronic kidney disease (CKD) show a chronic microinflammatory state that promotes premature aging of the vascular system. Currently, there is a growth interest in the search of novel biomarkers related to vascular aging to identify CKD patients at risk to develop cardiovascular complications. METHODS: Forty-five CKD patients were divided into three groups according to CKD-stages [predialysis (CKD4-5), hemodialysis (HD) and kidney transplantation (KT)]. In all these patients, we evaluated the quantitative changes in microRNAs (miRNAs), CD14+C16++ monocytes number, and microvesicles (MV) concentration [both total MV, and monocytes derived MV (CD14+Annexin V+CD16+)]. To understand the molecular mechanism involved in senescence and osteogenic transdifferentation of vascular smooth muscle cells (VSMC), these cells were stimulated with MV isolated from THP-1 monocytes treated with uremic toxins (txMV). RESULTS: A miRNA array was used to investigate serum miRNAs profile in CKD patients. Reduced expression levels of miRNAs-126-3p, -191-5p and -223-3p were observed in CKD4-5 and HD patients as compared to KT. This down-regulation disappeared after KT, even when lower glomerular filtration rates (eGFR) persisted. Moreover, HD patients had higher percentage of proinflammatory monocytes (CD14+CD16++) and MV derived of proinflammatory monocytes (CD14+Annexin V+CD16+) than the other groups. In vitro studies showed increased expression of osteogenic markers (BMP2 and miRNA-223-3p), expression of cyclin D1, ß-galactosidase activity and VSMC size in those cells treated with txMV. CONCLUSION: CKD patients present a specific circulating miRNAs expression profile associated with the microinflammatory state. Furthermore, microvesicles generated by monocytes treated with uremic toxins induce early senescence and osteogenic markers (BMP2 and miRNA-223-3p) in VSMC.

Mechanical tricuspid balloon 'valvuloplasty' in a prohibitive risk patient with cardiogenic shock: a case report.

BACKGROUND: We present a complex case of a failing tricuspid mechanical valve prosthesis in a patient with refractory cardiogenic shock at prohibitive risk for surgery in whom balloon 'valvuloplasty' resulted in immediate haemodynamic improvement in valve function. CASE SUMMARY: A 67-year-old woman with remote history of endocarditis s/p tricuspid valve repair and mechanical aortic valve replacement was referred for second opinion and management of new severe symptomatic tricuspid valve stenosis resulting in progressive debilitating congestive heart failure (HF). The patient was approved by the heart team to undergo redo open heart for surgical repair of the tricuspid valve. Intraoperative technical challenges were met to repair the tricuspid valve. In turn, the native valve was resected and a 33 mm On-X mechanical valve prosthesis. The patient's post-operative course was complicated by recurrent haemoptysis, prolonged mechanical respiratory support, acute kidney injury, and cardiogenic shock. Surgical re-exploration to address the dysfunctional mechanical tricuspid valve was felt to be prohibitive. Structural heart team was consulted. Cardiac catheterization was recommended to ascertain and confirm findings. The patient was transferred to the cardiac catheterization laboratory. Initial fluoroscopic examination of the heart confirmed the echocardiographic results of an immobile septal leaflet of the recently implanted mechanical tricuspid valve. An 8 × 40 mm Mustang OTW angioplasty balloon was then advanced across the mechanical valve and inflated gradually at nominal pressure. A single inflation resulted in successful restoration of valve leaflet function. DISCUSSION: To the best of our knowledge, this is the first balloon 'valvuloplasty' on a mechanical On-X valve in the tricuspid position.

Role of endothelial microvesicles released by p-cresol on endothelial dysfunction.

Protein bound uremic toxins, such as p-cresol, cannot be effectively removed by conventional dialysis techniques and are accumulated in plasma, thus contributing to progression of both chronic kidney disease (CKD) and cardiovascular disease (CVD). Pathological effects of uremic toxins include activation of inflammatory response, endothelial dysfunction and release of endothelial microvesicles. To date, the role of p-cresol in endothelial microvesicles formation has not been analyzed. The aim of the present study was evaluate the effects of endothelial microvesicles released by p-cresol (PcEMV) on endothelial dysfunction. An in vitro model of endothelial damage mediated by p-cresol was proposed to evaluate the functional effect of PcEMV on the endothelial repair process carried out by endothelial cells and microRNA (miRNA) that could be involved in this process. We observed that p-cresol induced a greater release of microvesicles in endothelial cells. These microvesicles altered regenerative capacity of endothelial cells, decreasing their capacity for cell migration and their potential to form vascular structures in vitro. Moreover, we observed increased cellular senescence and a deregulation of miRNA-146b-5p and miRNA-223-3p expression in endothelial cells treated with endothelial microvesicles released by p-cresol. In summary our data show that microvesicles generated in endothelial cells treated with p-cresol (PcEMV) interfere with the endothelial repair process by decreasing the migratory capacity, the ability to form new vessels and increasing the senescence of mature endothelial cells. These alterations could be mediated by the upregulation of miRNA-146b-5p and miRNA-223-3p.

Validación de la versión en español de la escala Perspectiva Espiritual de P. Reed / Validation of the Spanish Version of Reed's Spiritual Perspective Scale

Introducción: La espiritualidad es un fenómeno complejo, que en su concepción y experimentación está determinada por múltiples factores, lo cual complejiza el ejercicio de su evaluación en diferentes contextos. Objetivo: Analizar la estructura factorial de la versión en español de la Escala de Perspectiva Espiritual de Reed (1987) en una muestra chilena de adultos. Métodos: Investigación cuantitativa, evaluación psicométrica de un instrumento. El estudio se realizó durante el año 2016 en la ciudad de Antofagasta, Chile. Se realizó un análisis factorial confirmatorio, se utilizó el método de Máxima Verosimilitud Robusto. La muestra estuvo constituida por 174 adultos, cuyo promedio de edad fue de 23,3 años (DS = 5,003). Resultados: Se revela una estructura de dos factores: creencias y prácticas espirituales. Al liberar los errores de medición, los índices de bondad de ajuste dan cuenta de un ajuste excelente del modelo. Conclusiones: La escala de perspectiva espiritual de Reed (versión en español) entrega puntuaciones válidas al medir el nivel de espiritualidad en adultos, evaluando dos factores de la espiritualidad(AU)

Introduction: Spirituality is a complex phenomenon, which is, in its conception and experimentation, determined by multiple factors, which complicates the exercise of its evaluation in different settings. Objective: To analyze the factor structure of the Spanish version of the Spiritual Perspective Scale (Reed 1987) in a Chilean sample of adults. Methods: Quantitative research, psychometric evaluation of an instrument. The study was conducted during 2016 in the city of Antofagasta, Chile. Confirmatory factor analysis was used, together with the robust maximum likelihood method. The sample consisted of 174 adults, whose average age was 23.3 years (SD=5.003). Results: A structure of two factors is revealed: spiritual beliefs and practices. On releasing the measurement errors, the indices of goodness of fit show an excellent fit of the model. Conclusions: The Spanish version of Read's Spiritual Perspective Scale delivers valid scores upon measuring the level of spirituality in adults, evaluating two spirituality factors(AU)

Gas Accretion within the Dust Cavity in AB Aur.

AB Aur is a Herbig Ae star hosting a well-known transitional disk. Because of its proximity and low inclination angle, it is an excellent object to study planet formation. Our goal is to investigate the chemistry and dynamics of the molecular gas component in the AB Aur disk, and its relation with the prominent horseshoe shape observed in continuum mm emission. We used the NOEMA interferometer to map with high angular resolution the J = 3-2 lines of HCO+ and HCN. By combining both, we can gain insight into the AB Aur disk structure. Chemical segregation is observed in the AB Aur disk: HCO+ shows intense emission toward the star position, at least one bright molecular bridge within the dust cavity, and ring-like emission at larger radii, while HCN is only detected in an annular ring that is coincident with the dust ring and presents an intense peak close to the dust trap. We use HCO+ to investigate the gas dynamics inside the cavity. The observed bright HCO+ bridge connects the compact central source with the outer dusty ring. This bridge can be interpreted as an accretion flow from the outer ring to the inner disk/jet system proving gas accretion through the cavity.

Pancreatic autoantibodies and CD14+CD16+ monocytes subset are associated with the impairment of ß-cell function after simultaneous pancreas-kidney transplantation.

Pancreatic autoantibodies (AAb) has been associated with a worse pancreas graft survival after simultaneous pancreas-kidney transplantation (SPK). However, due to the variable time for AAb to become positive and the lack of early biomarkers suggesting such autoimmune activation, the mechanisms leading ß-cell destruction remain uncertain. The present study aimed to evaluate the association between post-transplant AAb and the functional impairment of the pancreatic ß-cell and also the association of such AAb with inflammation after SPK. In a longitudinal study, we analyzed the impact of post-transplant glutamic acid decarboxylase (GAD-65) and the insulinoma-associated autoantigen 2 (IA-2) AAb on pancreas graft function. Serum Hb1Ac and C-peptide (C-pep) were longitudinally compared between a group with positive posttransplant AAb (AAb+; n = 40) and another matched group with negative AAb (AAb-; n = 40) until the fifth year following seroconversion. In the cross-sectional analysis, we further evaluated the systemic signatures of inflammation by measuring pro-inflammatory CD14+CD16+ monocytes by flow-cytometry and interleukin 17-A serum levels in 38 SPK recipients and ten healthy controls. In the longitudinal study, patients with AAb+ showed higher levels of Hb1Ac (p<0.001) and lower C-pep levels (p<0.001) compared to those who remained AAb- throughout the follow-up. In the cross-sectional study, AAb+ patients showed a higher percentage of CD14+CD16+ monocytes compared with those with AAb- and the healthy controls (6.70±4.19% versus 4.0±1.84% and 3.44±0.93%; p = 0.026 and 0.009 respectively). Also, CD14+CD16+ monocytes correlated with Hb1Ac and C-pep serum levels. Multivariate logistic regression showed that posttransplant AAb+ was independently associated with a higher percentage of pro-inflammatory monocytes (adjusted-OR 1.59, 95%CI 1.05-2.40, p = 0.027). The group of patients with positive AAb also showed higher levels of IL17A as compared with the other groups (either healthy control or the negative AAb subjects). In conclusion, pancreatic AAb+ after SPK were not only associated with higher Hb1Ac and lower c-peptide serum levels but also with an increased percentage of CD14+CD16+ monocytes and higher levels of circulating IL17-A.

Efecto de los residuos fibrosos de avena ( Avena sativa ) y caraotas ( Phaseolus vulgaris ) sobre la actividad in vitro de las disacaridasas intestinales en ratas / Effect of the Fibrous Residues of Avena (Avena sativa) and Black Beans (Phaseolus vulgaris) on the in vitro Activity of Intestinal Disacaridases in Rats

Resumen Antecedentes: las disacaridasas intestinales pueden ser inhibidas o estimuladas parcialmente en presencia de fibra. Objetivo: evaluar el efecto de los residuos fibrosos de avena (Avena sativa) y caraotas (Phaseolus vulgaris) sobre la actividad in vitro de las disacaridasas intestinales. Materiales y métodos: 15 ratas Sprague Dawley se dividieron en tres grupos: un grupo control, un grupo alimentado con harina de caraota y un grupo alimentado con harina de avena, durante 21 días. Se obtuvo un homogeneizado de la mucosa intestinal que fue utilizado para la determinación de la actividad de las disacaridasas por un método enzimático, en presencia de sustrato natural y con la adición de residuos fibrosos de harina de avena y caraotas en concentración de 2,5 % (P/V). Resultados: la mayor actividad enzimática se registró en la región intestinal media para cada enzima (p<0,05). El orden de actividad enzimática en mg glucosa/mg proteína/min fue maltasa (0,149) sacarasa (0,096) y lactasa (0,014) (p<0,05). La maltasa fue inhibida en mayor medida por el residuo de caraota; la sacarasa, por el residuo de avena; y la lactasa, por ambos. Conclusiones: la adición de fibra purificada de avena y caraota produjo una disminución significativa de la actividad in vitro de las disacaridasas intestinales, especialmente en presencia del residuo de caraota.

Abstract Background: Intestinal disaccharidases can be partially inhibited or stimulated in the presence of fiber. Objective: To evaluate the effect of fibrous residues of oats (Avena sativa) and black beans (Phaseolus vulgaris) on the "in vitro" activity of the intestinal disaccharidases. Materials and Methods: 15 Sprague Dawley rats, were divided into three groups: control, fed with bean flour, and fed with oatmeal flour for 21 days. Homogenate was obtained by scraping the mucosa. The determination of enzymatic activity of the disaccharidases was measured by the enzymatic method, in the presence of its natural substrate and with addition of the fibrous residues obtained from the oatmeal and black beans, in concentration of 2.5 % (W/V). Results: The highest enzymatic activity was recorded in the middle intestinal region for each enzyme (p <0.05). The order of enzymatic activity in mg glucose / mg protein / min was maltase (0.149) sucrase (0.096) and lactase (0.014) (p<0.05). Maltase was inhibited to a greater extent by bean residue; sucrase by oat residue and lactase by both. Conclusion: The addition of purified fiber of oats and bean produced a significant decrease in the in vitro activity of the intestinal disaccharidases, especially in the presence of the bean residue.

La corrección del déficit de 25-OH-vitamina D mejora el control del hiperparatiroidismo secundario y el estado inflamatorio de pacientes estables en hemodiálisis / Correction of 25-OH-vitamin D deficiency improves control of secondary hyperparathyroidism and reduces the inflammation in stable haemodialysis patients

Introducción: El déficit de 25-OH-vitamina D (25-OH-D) es común en los pacientes en hemodiálisis (HD). Por otra parte, es bien conocida la elevada incidencia de hiperparatiroidismo secundario en este grupo de pacientes, y lo importante que es su adecuado control. La 25-OH-D está implicada en la regulación de la homeostasis del calcio, por lo que tener niveles adecuados puede contribuir en el control del metabolismo óseo-mineral. Objetivos: Evaluar el efecto de la repleción de 25-OH-D en pacientes en HD con déficit vitamínico (niveles<20ng/ml), en el control del hiperparatiroidismo secundario y en el estado de microinflamación. Pacientes y métodos: Estudio observacional, prospectivo en el que se trataron pacientes estables en HD con déficit de 25-OH-D (<20ng/ml), con calcifediol 0,266mcg/15 días vía oral durante 3 meses. Los datos de HD, parámetros bioquímicos y las dosis de fármacos administrados fueron analizados antes y después de la corrección del déficit. Resultados: Un total de 45 pacientes estables en HD con edad media 74,08±12,49 años completaron el tratamiento. Del total, 27 pacientes (60%) alcanzaron niveles de 25-OH-D>20ng/ml (en 23 fueron>30ng/ml, y 4 entre 20-30ng/ml). Las cifras de hormona paratiroidea descendieron en 32 de los 45 pacientes, alcanzando en 23 (51% de tratados) un descenso>30% respecto al valor basal. En cuanto al tratamiento concomitante, se objetivó un descenso significativo de la dosis de activador selectivo del receptor de vitamina D; sin evidenciarse cambios en la dosis de calcimimético ni de quelantes. Respecto al estado de malnutrición-inflamación, destaca un descenso de la proteína C reactiva, aunque no se modificaron otros parámetros de microinflamación como los monocitos activados (CD14+/CD16+ y CD 14++/CD16+). Tampoco se observaron cambios en los niveles de FGF-23. Conclusiones: La corrección del déficit de 25-OH-D en pacientes en HD se asocia a un mejor control del hiperparatiroidismo secundario con menores dosis de análogos de vitamina D y a una mejoría en el estado inflamatorio de estos pacientes. Nuestros resultados apoyan la recomendación de determinar niveles de 25-OH-D y corregir el déficit en pacientes en HD (AU)

Introduction: Patients on haemodialysis (HD) have a high prevalence of 25-OH-vitamin D (25-OH-D)deficiency. Secondary hyperparathyroidismis a common condition in these patients, which is very important to control. 25-OH-D is involved in regulating calcium homeostasis. As such, appropriate levels of this vitamin could help to control bone mineral metabolism. Objective: To evaluate the effect 25-OH-D repletion in HD patients with 25-OH-D deficiency (<20ng/ml) on the control of secondary hyperparathyroidism and microinflammation status. Patients and methods: Prospective observational study in which stable patients on HD with 25-OH-D deficiency (<20ng/ml) were treated with oral calcifediol 0.266mcg/every 2 weeks for three months. Dialysis characteristics, biochemical parameters and drug doses administered were analysed before and after the correction of the deficiency. Results: Forty-five stable HD patients with a mean age of 74.08±12.49 years completed treatment. Twenty-seven patients (60%) achieved 25-OH-D levels above 20ng/ml (23 with levels>30ng/ml and 4 between 20-30ng/ml). Parathyroid hormone levels decreased in 32 of the 45 patients, 23 of which (51%) achieved a>30% decrease from baseline. In terms of concomitant treatment, we observed a significant reduction in the selective vitamin D receptor activator dose, but no changes in calcimimetic or phosphate binders administration. In terms of malnutrition-inflammation status, a decrease in C-reactive protein was noted, although other microinflammation parameters, such as activated monocytes (CD14+/CD16+ and CD 14++/CD16+) were unchanged. No changes were observed in the levels of FGF-23. Conclusions: Correcting 25-OH-D deficiency in HD patients is associated with better secondary hyperparathyroidism control with lower doses of vitamin D analogues, as well as an improvement in inflammatory status. Our results support the recommendation to determine 25-OH-D levels and correct its deficiency in these patients (AU)